The phenomenon known as ‘fluorescence’ is now widely used in the chemical, physical and life sciences largely due to the development of highly sophisticated fluorescent probe chemistries and the commercial availability of these probes as well as the development of novel microscopy approaches. This Second Edition of Introduction to Fluorescence helps readers acquire a thorough understanding of basic fluorescence theory and practice. It describes the general principles in a direct way and uses examples from a variety of disciplines to demonstrate them. Since the First Edition, significant advances in the field have appeared. For example, phasors, both lifetime and spectral phasors, have become very popular, and so a new chapter dedicated to this topic has been added in this edition. Furthermore, significant advances have been made in fluorescence microscopy methods, including super-resolution and single-molecule techniques. In color throughout, the book takes readers through the history
